Volume 2, Issue 1 (2021)

Farah Junaid, Laiba Ali Khawaja, Syeda Zara Kazmi and Hamid Mukhtar^*

Institute of Industrial Biotechnology, Government College University Lahore-54000, Pakistan.

*Corresponding Author:

Hamid Mukhtar
Institute of Industrial Biotechnology, Government College University Lahore-54000, Pakistan.
Tel: +92 333 4245581, +92 42 99211634;E-mail: hamidwaseer@yahoo.com

Received date: December 24, 2020; Accepted date: January 18, 2021; Published date: January 25, 2021

Citation: Junaid F, Khawaja LA, Kazmi SZ, Mukhtar H (2020) Purification of Azoreductase for Industrial Applications: A Review. Int J Biol Chem Res. 2021;2(1):1-10.

Copyright: © 2021 Junaid F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Bacterial azoreductases were exploited for research purposes to assess their potential to degrade azo dyes. Azo dyes are toxic and needs to be removed, degraded converted into less toxic forms. Various bacterial strains isolated from the azo-dye contaminated soils, industrial effluent discharge, waste water etc. were the potential source for their isolation, recombinant DNA technology has enabled to increase the expression of azo-dye degrading genes in different expression systems to be used in industries. The demand to purification varies upon the potential use of the azoreductase in industries keeping in view their effects on human health. This review article is concerned with the purification strategies which are involved in extraction (in case of intracellular and membrane bound enzyme) and purification of bacterial azoreductases along with their activity and toxicity assays.

Keywords

Xenobiotics; Flavoenzymes; Ion-exchange chromatography; Hydrophobic interaction chromatography; Azo dyes